BIOspektrum Fernstudium

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Hier finden Sie "Appetithäppchen" aus dem aktuellen BIOspektrum-Heft.


Ubiquitin-Ligasen: heiße Targets für die Wirkstoffentwicklung?

 (S. 244)
Lena Ries, Sonja Lorenz

Ubiquitin regulates countless physiological and pathophysiological processes and, as a result, ubiquitination enzymes have emerged as key targets on the drug discovery arena. Here we discuss recent progress in the development of small-molecule inhibitors directed at ubiquitin ligases and highlight novel strategies that may pave the way for rational drug design approaches.

Die Sensorik der Zelle für die Biotechnologie nutzen

 (S. 247)
Julia Frunzke

VAAM-Forschungspreis 2016
Nature has evolved a plethora of different sensor devices in form of proteins or RNAs. In recent studies, we have harnessed the sensory capability of bacterial transcription factors for the design of genetically encoded biosensors enabling the detection of a variety of different intra- or extracellular stimuli. These biosensors provide important insights into microbial population dynamics and can be implemented in high-throughput screening approaches accelerating biotechnological strain development.

Bakterielle Kommunikation: Signale und Signal-inaktivierende Enzyme

 (S. 251)
Franziska S. Birmes, Susanne Fetzner

Bacteria are social organisms capable of collective behaviors mediated by chemical communication or “quorum sensing” (QS). However, QS signal molecules often have multiple biological functions. Because many bacteri al pathogens regulate virulence gene expression via QS, these systems are attractive targets for antivirulence therapies. One possible strategy to interfere with QS is signal inactivation by quorum quenching enzymes.

Funktionales Proteom-Display zur Identifikation von Biomarkern

 (S. 256)
Stefan Dübel, Jonas Zantow, Michael Hust

Proteome display allows the functional display of positive selected open reading frames (ORF) of randomly fragmented whole genomes or even metagenomes, thus covering all possible proteins encoded by a pathogen or an entire microbiome. Immunogenic proteins can then be selected from these libraries using patient-derived immunoglobulins (serum). Hence, proteome display allows the identification of immunogenic proteins even if they are only expressed in the host but not in cultivation, as well as the detection of very low expressed and very small immunogens.

Fluoreszierende Proteinsensoren für die Redoxregulation in lebenden Zellen

 (S. 260)
Bruce Morgan, Markus Schwarzländer

Redox signalling is increasingly recognized to play a crucial role in cellular and organismal physiology. Whilst we have made considerable advances in recent years with respect to understanding the roles and regulation of many different cellular redox species, research remains restricted by a lack of appropriate tools and techniques. Here we discuss the opportunities afforded by recently developed genetically encoded redox sensors and illustrate how they have already advanced our understanding.

qPCR zur quantitativen Validierung von Metagenomdaten

 (S. 265)
Michael Schloter, Stefanie Schulz, Fabian Bergkemper, Maria de Vries, Anne Schöler

Microorganisms are essential to maintain ecosystem functions and health as they catalyze most keystone processes. Formerly the quantification of key functions was performed by broad ranged primers, which were mainly based on genomes of cultivated microbes. To take advantage of the enormous development of next generation sequencing technologies, we propose to use metagenomic data for the design of new primer systems. Here we describe a two-phasic approach for a targeted and site specific primer design for qPCR.

PCR-basierte Methoden für den Erregernachweis bei Dermatophytosen

 (S. 270)
Christiane Kupsch, Yvonne Gräser

It is widely accepted that the use of molecular detection methods can improve the diagnosis of bacterial and fungal infections in terms of time, expenditure and sensitivity when compared to conventional identification, based on microscopic examination and cultivation of clinical specimens. Here we describe the capabilities and challenges of DNA-based diagnostics on the example of detecting and differencing infectious agents of dermatophytosis.

Anwendungen & Produkte

Quarzkristall-Mikrowaagen-Technologie als neue bioanalytische Plattform

 (S. 284)
Paula Braun, Hartmut Drechsel, Albert Sterck, Jin Zhang, Gudrun Prepens, Thomas Reiner, Frank K. Gehring

QCM-D is a surface-sensitive technique for real-time monitoring of biolayers on surfaces with regard to adsorption and desorption, molecular interactions and structural properties. To date, this old-established technique is employed for studies of cellular processes, both, in nanotechno - lo gy and cell biology. Interactions among molecules, cells or between cells and the environment are observable in their natural unaltered states. QCM-D thus provides a unique insight into the complex world of biology.


Biogene Eisenminerale kontrollieren das Umweltverhalten toxischer Metalle

 (S. 316)
E. Marie Muehe, Andreas Kappler

Some microorganisms oxidize iron(II) and reduce iron(III) for energy gain and growth. These redox processes lead to the formation of microbially formed (biogenic) iron minerals, which are small and have a large surface area for the sorption of other metals. Here, we provide an overview of microbially catalyzed iron redox reactions and the properties of biogenic iron minerals and discuss the consequences of biogenic mineral formation for the environmental fate of toxic metals.



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Aktuell: PCR/qPCR

Aktuell: PCR/qPCR

Die Polymerase Chain Reaction, kurz PCR, ermöglicht den hochspezifischen Nachweis von winzigsten Mengen an DNA. Damit hat diese Methode seit ihrer Etablierung in den 1980er Jahren sowohl die Molekularbiologie als auch ihre Anwendungen in der medizinischen Diagnostik revolutioniert. Die Analyse von Verwandtschaft – nicht nur in Vaterschaftsfragen sondern auch zwischen Arten, oder das Erkennen von Erbkrankheiten oder Virusinfektionen stehen hier beispielhaft für zahlreiche spannende Fragestellungen. (Hintergrundbild: Eppendorf twin.tec® PCR Plates; mit freundlicher Genehmigung der Eppendorf AG,

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Kompakter CO<sub>2</sub>-Inkubator

Kompakter CO2-Inkubator

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Aktuell: Microplate Reader

Hier finden Sie alle Marktübersichten aus den Jahren 2005 bis 2016.
In 2016 erscheinen 2 Übersichten: Microplate Reader (2/16) und Liquid Handler und Dispenser (6/16).
Zuletzt erschienen ist 2/16: Microplate Reader.

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