Automatisierte Analyse der Koloniebildung

Abstract

The colony formation assay evaluates the proliferative capacity of a
single cell. For applications such as cancer drug screening, it is important to distinguish cells that retain this proliferative capacity from
those that do not. Conventional analysis of this assay involves scoring and quantifying colonies in each well of a multi-well format manually by eye, limiting its throughput capabilities. We present an automated method for conducting and analyzing the colony formation assay in
both a 6-well and 96-well microplate using the upright color brightfield imaging with a wide field of view.