Bacteria have been extensively investigated in their unicellular mode of
life in liquid cultures. However, biofilm communities are now considered
to be the most abundant mode of bacterial life on Earth, and many properties
and functions of biofilms are still unclear. Mechanistically understanding
which properties and functions of biofilms emerge at the singlecell
level, and which emerge at the multicellular level, presents a major
challenge for biofilm research in the coming years.