Computergestütztes Design mikrobieller Zellfabriken


A key principle for the rational design of cell factories is the stoichio -
metric coupling of growth and product synthesis. Based on this approach
we recently constructed an Escherichia coli strain producing itaconic acid
with excellent yields. Furthermore, in a large-scale computational study
we demonstrated that coupling of growth and production is, in principle,
feasible for almost all metabolites in five major production organisms.
These results are of fundamental importance for rational metabolic
engineering in biotechnology.