Expression und Untersuchung von Pflanzenproteinen mit Leishmania tarentolae

Abstract

Glycosylation is an important post-translational modification that can be
found in many eukaryotic proteins. Since it can be crucial for protein
structure and function, properly glycosylated proteins are of paramount
importance for crystal structure determination. The eukaryotic expression
system LEXSY, based on the protozoan Leishmania tarentolae, has
been used to express and crystallize fully glycosylated Arabidopsis
thaliana
legumain γ and elucidate the role of glycosylation for its function.

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