GPCR-Aktivierung: Live-Berichterstattung aus der Zelle

Abstract

Live cell assays with genetically encoded, fluorescent biosensors provide
significant advantages over endpoint assays in cell lysates as they
acquire the timing of cellular responses in cells relevant to disease. Here
we show how second messengers relevant to the Gs/Gi or the Gq signalling
pathway are detected in living cells using assays for cAMP, diacylglycerol
(DAG), and Ca2+.