Hochaufgelöste Visualisierung einzelner Moleküle auf ganzen Zellen


Biological systems are dynamic and three-dimensional but many techniques allow only static and two-dimensional observation of cells. We
used three-dimensional (3D) lattice light-sheet single-molecule localization microscopy (dSTORM) to investigate the complex interactions
and distribution of single molecules in the plasma membrane of whole cells. Different receptor densities of the adhesion receptor CD56 atdifferent parts of the cell highlight the importance and need of three-dimensional observation and analysis techniques.