Höchstmögliche räumlich-zeitliche Auflösung in der Lichtmikroskopie


Super resolution (SR) fluorescence microscopy techniques STED, SIM and
PALM/STORM have revolutionized light microscopy and provide resolution
of imaging way below the general limit of resolution as defined by the
Abbe law. Single molecule tracking (SMT) allows the pursuit of movement
of single proteins or lipids in real time, revealing in vivo dwell times and
binding kinetics, a further milestone in live cell imaging. A combination of
SR and SMT is a logical next evolution step in the goal to study dynamic
processes in live cells at a maximal spatial and temporal resolution.