Kofaktor-Mikroarrays zur Identifikation von Kernrezeptorliganden

Abstract

Different in vitro approaches exist to identify agonistic or antagonistic
activity of chemical substances concerning nuclear receptors, since
adverse effects in vivo can be the consequence. The recruitment of
coregulators by nuclear receptors is a key step in the ligand-induced
transcription of controlled genes. Therefore, the microarray assay for
real-time coregulator-nuclear receptor interaction (MARCoNI) was tested
in combination with a reporter cell assay as a screening tool in toxicology.