At-line Monitoring von Zellkulturprozessen mittels Massenspektrometrie


Perfusion becomes a reasonable alternative
to traditional batch or fed-batch processes,
partly due to the increasing availability of
suitable re-usable or single-use equipment.
High cell densities and long duration of perfusion
processes require reliable process
control. Amongst others, monitoring of cell
stress becomes important. Intact cell MALDITOF
mass spectrometry (ICM-MS) biotyping
is described here as a robust at-line monitoring
method of cell stress in perfusion cell

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