Messung der Bindungsaffinität von Protein-Protein-Interaktionen

Abstract

A new technology, microfluidic diffusional sizing (MDS), can be used to
assess the binding affinity of proteins in both simple and complex backgrounds
with high precision. Whereas most techniques for quantitative
analysis of protein-protein interactions require lengthy purification, MDS
can avoid this often complex step, opening up the possibility to investigate
difficult-to-study proteins. It also means that detailed binding analyses
can be performed earlier, avoiding purification and providing insight
sooner.