Mischen von Tröpfchen im Flug zum ortsaufgelösten 3D-Druck von Hydrogelen

Abstract

Piezoelectric dispensers are widely used for microarrays. We have
developed a “twin pipette” head to mix two droplets of reactive polymer
solutions in flight and on the target. Two dispensers can pivot to either a
straight (for sample uptake, washing) or an inclined position (for dispensing).
We show how two precursor solutions, with or without cells, cross -
link immediately after printing, allowing for complex 3D cell cultures in
spatially graded hydrogels with structure sizes ≥ 50 μm.