Nucleotid-genaue PCR: von der Mutationsdetektion zur Genom-Editierung

Abstract

A frequently used method for the analysis of single nucleotide varia- tions in DNA is allele-specific PCR (asPCR). AsPCR poses major chal- lenges to the DNA polymerase used for this purpose e. g., the avoid- ance of the formation of false results due to the formation of side products. Several independently conducted studies show that a highly discriminative mutant of Taq DNA polymerase is ideally suited for usein asPCR in numerous research areas ranging from mutation detectionto genome editing.

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