Probennahme per Laserablation von Krebsgeweben für Proteomics

Abstract

Distant metastasis is the major cause of cancer-related
death in virtually all tumour types. We used spontaneous
metastasis xenograft mouse models to study
differences between primary tumours and corresponding
metastases at the proteome level. Sampling of
tissues for proteomics with mechanical homogenization
is critical, because by this process many proteins
are getting lost. For improving the protein yield
infrared laser ablation was applied for sampling.