Protein-AMPylierungs-Identifikation in lebenden Zellen

Abstract

Protein AMPylation is a prevalent protein post-translational modification in human cells involved in endoplasmic reticulum stress regulation and neural development. In this article we describe the design, synthesis and application of a pronucleotide probe suitable for in situ fluorescence imaging and chemical protemics profiling of AMPylated proteins. Our probe utilizes straightforward strain-promoted azide-alkyne click reaction for fluorescence labeling in living cells.

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