Protein-Cross-Linking zur Aufklärung von komplexen Strukturen


Cryo-electron microscopy (cryo-EM) can solve structures of highly dynamic
macromolecular complexes. To characterize less well defined regions in
cryo-EM images, cross-linking coupled with mass spectrometry (CX-MS)
provides valuable information on the arrangement of domains and amino
acids. CX-MS involves covalent linkage of protein residues close to each
other and identifying these connections by mass spectrometry. Here, we
summarise the advances of CX-MS and its integration with cryo-EM for
structural reconstruction.