Pyrosequenzierung zur molekularen Altersschätzung in der DNA-Spurenanalyse


The estimation of the chronological age based on DNA methylation
markers by bisulfite sequencing is a promising new method in forensic molecular genetics. The application of the method to forensic trace
samples from crime scenes was recently legalized following a change of the German law. Biological traces display a challenging sample
source since they are usually of small amount. To increase sensitivity, we have developed an optimized pyrosequencing protocol for quantitative methylation analysis.