Pyrrolysyl-tRNA-Synthetase: Methanogenese und Gencode-Erweiterung


Pyrrolysyl-tRNA synthetase (PylRS) is an enzyme of some methanogenic Archaea for the natural  ncorporation of pyrrolysine into proteins. The discovery of PylRS as a natural tool for genetic code expansion
paved the way for site-specific incorporation of non-canonical amino acids (ncAAs) into proteins, with versatile side chains useful in biotechnology. Almost 20 years after the discovery, we describe the journey which led to three distinct classes of PylRSs with unique ncAA recognitions.