RNA-Live-Imaging in Mikroorganismen

Abstract

RNA live imaging is a powerful technique to visualize the subcellular
localization as well as highly dynamic transport of mRNAs in vivo.
Established in eukaryotic microorganisms, it is now a wide-spread
system used in bacteria, animals, and plants. The method is based on
the binding of heterologous RNA-binding proteins to their cognate
binding sites that are integrated into the mRNA of interest. Recent
advances enable the visualization of transcriptional or translational
sites as well as mRNA decay.

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