RNA-Live-Imaging in Mikroorganismen


RNA live imaging is a powerful technique to visualize the subcellular
localization as well as highly dynamic transport of mRNAs in vivo.
Established in eukaryotic microorganisms, it is now a wide-spread
system used in bacteria, animals, and plants. The method is based on
the binding of heterologous RNA-binding proteins to their cognate
binding sites that are integrated into the mRNA of interest. Recent
advances enable the visualization of transcriptional or translational
sites as well as mRNA decay.