„Schiffe Versenken“: Optimierung einer hochaktiven Saccharose-Isomerase


The sucrose isomerase SmuA fromSerratia plymuthica catalyses the production of isomaltulose, an artificial sweetener used in the food industry. To suppress the formation of the hygroscopic by-product trehalulose we applied a semirational protein engineering strategyinspired by the “battleship” board game. After seven cycles of introducing amino acid exchanges around the active site and investigating their influence on the enzymatic product profile we obtained a triplemutant that showed 2.3 times less trehalulose formation but had retained high catalytic efficiency.