Single-particle tracking von GPCRs


Single-particle tracking (SPT) is a method of high-resolution microscopy
to investigate the dynamics of single molecules inside cells or
on the cell surface. Here we describe for the first time the applicability
of the HiBiT Protein Tagging System combined with the HaloTag®
self-labeling protein technology for monitoring the lateral diffusion of
a pharmacological relevant G protein-coupled receptor (GPCR) by SPT.