Systemweite Anreicherung von RNA-Protein-Komplexen


RNA-protein complexes (RNPs) are key players in cell physiology,
especially in the context of RNA metabolism. However, their analysishas been dependent on specific protein epitopes or RNA sequence
elements, preventing unbiased and cell-wide studies. We developed a three-step protocol, termed Phenol Toluol extraction (PTex), to isolatethe complete suite of RNPs in cells, solely based on their unique physicochemical properties after UV-cross-linking. PTex, along withother recently developed unbiased techniques, has the potential toguide the way to cell-wide analysis of RNA-protein interactions.