Untersuchung von molekularen Mechanismen der Virusreplikation in Echtzeit

Abstract

Viral replication is a crucial step in the virus life cycle and for the determination of its pathogenicity and can be investigated in a microplate assay by monitoring viral gene expression. As an illustra-tive example, the Sindbis virus (SINV, Alphavirus genus) with an engineered genome containing the coding sequence for mCherry fluorescent protein was used. Expression of mCherry can be used as aproxy for viral gene expression and can be measured at regular inter-vals throughout the infection to simulate viral replication.

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