Von der „Blobology“ zur atomaren Auflösung der Kryo-Elektronenmikroskopie


It took almost a century to develop electron microscopy into a powerful method for high-resolution structure determination of proteins.
Technical improvements in microscopy, detector technology, and
image processing software contributed to the exponential growth of
high-resolution structures of protein complexes determined by
cryo-electron microscopy in recent years. We now succeeded in breaking another resolution barrier in cryo-electron microscopy and for the first time in achieving true atomic resolution, where single atoms inthe protein can indeed be visualized individually. These improvementsin cryo-EM indicate that the method will continue to gain importance, not only as a method for structure determination but also in the development of new drugs in pharmaceutical research.