Von der Wurzel ins Labor: Meerrettichperoxidase produziert in E. coli

Abstract

The enzyme Horseradish Peroxidase (HRP) is omnipresent in modern biotechnology. Although promising for therapeutic purposes, no suitable production process for this enzyme has been available until now. Medical applications require the enzyme to be highly pure, homogenous
and well-defined. We have developed an efficient production process for recombinant HRP from Escherichia coli inclusion bodies. With this strategy we are able to provide active, highly pure and non-glycosylated enzyme at competitive yields.

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